Mass spectroscopic analysis (MS) could be a powerful analytical tool with several applications in the pharmaceutical and medical speciality field. The rise in sensitivity and determination of the instrument has opened new dimensions in the analysis of prescribed drugs and complicated metabolites of biological systems. Compared with alternative techniques, spectrum analysis is barely the technique for relative molecular mass determination, through that, we are able to predict the formula. It supported the conversion of the sample into an ionizing state, with or while not fragmentation that is then known by their mass-to-charge ratios (m/e). Spectrometry analysis provides made elemental info that is a crucial plus to interpret advanced mixture parts. Thus, it's a crucial tool for the structure elucidation of unknown compounds. Spectrometry analysis conjointly helps in quantitative elemental analysis, that is, the intensity of a mass spectra signal is directly proportional to the share of the corresponding part. It’s conjointly a noninvasive tool that allows in vivo studies in humans. Recent analysis has looked into the potential applications of mass spectrometers in a medical speciality field. It’s conjointly used as a sensitive detector for natural action techniques like LC-MS, GC–MS and LC/MS/MS. These recent combined technological developments of the technique have considerably improved its pertinency in pharmaceutical and medical speciality analyses.