El Alami M. has completed her PhD at the age of 30 years from University of Valencia, Spain. She has 9 publications. She is a doctor of pharmacy and has a master degree on pharmacology. She speaks 5 languages.
Statement of the problem: The key hallmark of stem cells is their ability to self-renew while keeping a differentiation potential. Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. Very much importance was given to the balance of nutrients, growth factors and pH buffers used to grow stem cells in vitro, little importance was given to the oxygen concentration in the culture media, as it was assumed that the ambiental 21% O2 was adequate for cell growth. Oxygen concentration has been closely linked to the maintenance of stemness and the widely used environmental 21% O2 concentration represents a hyperoxic non-physiological condition, that can impair stem cell behaviour by many mechanisms.
The aim of this study: Minimizing the time obtention of a large amount of viable dental pulp stem cells (DPSC) as critically important for stem cell banking for clinical use and to study the effect of the usual, high oxygen concentration (21% O2), compared to the physiological (3% O2), on the parameters of proliferation, oxidative stress and cell cycle regulation.