Marya El Alami
University of Valencia, Spain

Title: Relevance of oxygen concentration in stem cells culturing in vitro
Time: 14:50 - 15:20

Biography

El Alami M. has completed her PhD at the age of 30 years from University of Valencia, Spain. She has 9 publications. She is a doctor of pharmacy and has a master degree on pharmacology. She speaks 5 languages.

Research Interest


Abstract

Statement of the problem: The key hallmark of stem cells is their ability to self-renew while keeping a differentiation potential. Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. Very much importance was given to the balance of nutrients, growth factors and pH buffers used to grow stem cells in vitro, little importance was given to the oxygen concentration in the culture media, as it was assumed that the ambiental 21% O2 was adequate for cell growth. Oxygen concentration has been closely linked to the maintenance of stemness and the widely used environmental 21% O2 concentration represents a hyperoxic non-physiological condition, that can impair stem cell behaviour by many mechanisms.


The aim of this study: Minimizing the time obtention of a large amount of viable dental pulp stem cells (DPSC) as critically important for stem cell banking for clinical use and to study the effect of the usual, high oxygen concentration (21% O2), compared to the physiological (3% O2), on the parameters of proliferation, oxidative stress and cell cycle regulation.


Finding: Our results showed that the ambiental oxygen tension is far not the optimal oxygen condition for in vitro stem cells culturing, as it effects negatively the adhesion, proliferation rate, differentiation, oxidative stress, cell cycle regulation and antioxidative defense parameters. We also proposed the underlying molecular mechanism behind these results.